PrimePCR™ Probe Assay: PLEKHG3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029460
Assay Design:   Intron-spanning
Chromosome Location:   14:65209036-65209774question
Amplicon Length:   183
Splice Variants Targeted:   ENST00000247226 ENST00000394691 ENST00000471182 ENST00000484731

Gene Information

Description Not Available

Gene Symbol:   PLEKHG3
Gene Name:   pleckstrin homology domain containing, family G (with RhoGef domain) member 3
Aliases:   ARHGEF43, KIAA0599, MGC156299, MGC156300
RefSeq:   NC_000014.8 NG_016202.1 NT_026437.12
Ensembl:   ENSG00000126822
Entrez:   26030
UniGene:   Hs.509637
Chromosome Mapping:   14q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997500
y-intercept 35.370000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download