This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: PIM1, Human
PrimePCR™ Template for Probe Assay: PIM1, Human
The protein encoded by this gene belongs to the Ser/Thr protein kinase family and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells contributing to both cell proliferation and survival and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805 1825810).[provided by RefSeq Aug 2011]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.