PrimePCR™ Probe Assay: PIM1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029454
Assay Design:   Intron-spanning
Chromosome Location:   6:37140867-37141837question
Amplicon Length:   180
Splice Variants Targeted:   ENST00000373509

Gene Information

The protein encoded by this gene belongs to the Ser/Thr protein kinase family and PIM subfamily. This gene is expressed primarily in B-lymphoid and myeloid cell lines and is overexpressed in hematopoietic malignancies and in prostate cancer. It plays a role in signal transduction in blood cells contributing to both cell proliferation and survival and thus provides a selective advantage in tumorigenesis. Both the human and orthologous mouse genes have been reported to encode two isoforms (with preferential cellular localization) resulting from the use of alternative in-frame translation initiation codons the upstream non-AUG (CUG) and downstream AUG codons (PMIDs:16186805 1825810).[provided by RefSeq Aug 2011]

Gene Symbol:   PIM1
Gene Name:   pim-1 oncogene
Aliases:   PIM
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000137193
Entrez:   5292
UniGene:   Hs.81170
Chromosome Mapping:   6p21.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 36.640000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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