PrimePCR™ Probe Assay: DPCD, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029445
Assay Design:   Intron-spanning
Chromosome Location:   10:103354454-103361004question
Amplicon Length:   182
Splice Variants Targeted:   ENST00000370151 ENST00000370147 ENST00000434727 ENST00000370148 ENST00000370149

Gene Information

Description Not Available

Gene Symbol:   DPCD
Gene Name:   deleted in primary ciliary dyskinesia homolog (mouse)
Aliases:   DKFZp566F084
RefSeq:   NC_000010.10 NG_008091.1 NG_017063.1 NT_030059.13
Ensembl:   ENSG00000166171
Entrez:   25911
UniGene:   Hs.658128
Chromosome Mapping:   10q24.32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.840000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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