PrimePCR™ Probe Assay: NHSL1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029429
Assay Design:   Intron-spanning
Chromosome Location:   6:138794450-138817461question
Amplicon Length:   197
Splice Variants Targeted:   ENST00000427025 ENST00000343505 ENST00000342260 ENST00000491526 ENST00000533765

Gene Information

Description Not Available

Gene Symbol:   NHSL1
Gene Name:   NHS-like 1
Aliases:   C6orf63, KIAA1357
RefSeq:   NC_000006.11 NT_025741.15
Ensembl:   ENSG00000135540
Entrez:   57224
UniGene:   Hs.652741
Chromosome Mapping:   6q23.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.670000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download