PrimePCR™ Probe Assay: FAM53C, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029398
Assay Design:   Intron-spanning
Chromosome Location:   5:137681164-137682464question
Amplicon Length:   179
Splice Variants Targeted:   ENST00000434981 ENST00000239906 ENST00000513056

Gene Information

Description Not Available

Gene Symbol:   FAM53C
Gene Name:   family with sequence similarity 53, member C
Aliases:   C5orf6
RefSeq:   NC_000005.9 NT_034772.6
Ensembl:   ENSG00000120709
Entrez:   51307
UniGene:   Hs.728171
Chromosome Mapping:   5q31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.510000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download