PrimePCR™ Probe Assay: MYOC, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029371
Assay Design:   Intron-spanning
Chromosome Location:   1:171605750-171607841question
Amplicon Length:   175
Splice Variants Targeted:   ENST00000037502 ENST00000357746 ENST00000536591 ENST00000537133

Gene Information

MYOC encodes the protein myocilin which is believed to have a role in cytoskeletal function. MYOC is expressed in many occular tissues including the trabecular meshwork and was revealed to be the trabecular meshwork glucocorticoid-inducible response protein (TIGR). The trabecular meshwork is a specialized eye tissue essential in regulating intraocular pressure and mutations in MYOC have been identified as the cause of hereditary juvenile-onset open-angle glaucoma. [provided by RefSeq Jul 2008]

Gene Symbol:   MYOC
Gene Name:   myocilin, trabecular meshwork inducible glucocorticoid response
Aliases:   GLC1A, GPOA, JOAG, JOAG1, TIGR, myocilin
RefSeq:   NC_000001.10 NG_008859.1 NT_004487.19
Ensembl:   ENSG00000034971
Entrez:   4653
UniGene:   Hs.436037
Chromosome Mapping:   1q23-q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 34.510000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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