PrimePCR™ Probe Assay: RAB32, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029348
Assay Design:   Intron-spanning
Chromosome Location:   6:146865111-146870676question
Amplicon Length:   194
Splice Variants Targeted:   ENST00000367495

Gene Information

Small GTP-binding proteins of the RAB family such as RAB32 play essential roles in vesicle and granule targeting (Bao et al. 2002 [PubMed 11784320]).[supplied by OMIM Aug 2009]

Gene Symbol:   RAB32
Gene Name:   RAB32, member RAS oncogene family
Aliases:   Not Available
RefSeq:   NC_000006.11 NT_025741.15
Ensembl:   ENSG00000118508
Entrez:   10981
UniGene:   Hs.287714
Chromosome Mapping:   6q24.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997100
y-intercept 34.820000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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