PrimePCR™ Probe Assay: SMC4, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029019
Assay Design:   Intron-spanning
Chromosome Location:   3:160120589-160122213question
Amplicon Length:   135
Splice Variants Targeted:   ENST00000357388 ENST00000472991 ENST00000467468 ENST00000469762 ENST00000489573 ENST00000462787 ENST00000485867 ENST00000344722 ENST00000360111 ENST00000392788

Gene Information

Members of the structural maintenance of chromosomes or SMC family (e.g. SMC1A; MIM 300040) are critical for mitotic chromosome condensation in frogs and for DNA repair in mammals.[supplied by OMIM Nov 2010]

Gene Symbol:   SMC4
Gene Name:   structural maintenance of chromosomes 4
Aliases:   CAPC, SMC4L1, hCAP-C
RefSeq:   NC_000003.11 NG_022932.1 NT_005612.16
Ensembl:   ENSG00000113810
Entrez:   10051
UniGene:   Hs.58992
Chromosome Mapping:   3q26.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.530000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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