PrimePCR™ Probe Assay: KAZALD1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0029010
Assay Design:   Intron-spanning
Chromosome Location:   10:102822734-102824057question
Amplicon Length:   137
Splice Variants Targeted:   ENST00000370200 ENST00000313664

Gene Information

This gene encodes a secreted member of the insulin growth factor-binding protein (IGFBP) superfamily. It contains an N-terminal insulin growth factor-binding domain a central Kazal-type serine protease inhibitor and follistatin-like domain and a C-terminal immunoglobulin-like domain. Studies of the mouse ortholog suggest that this gene product may have a function in bone development and bone regeneration. [provided by RefSeq Feb 2009]

Gene Symbol:   KAZALD1
Gene Name:   Kazal-type serine peptidase inhibitor domain 1
Aliases:   BONO1, FKSG28, FKSG40, FLJ24094, IGFBP-rP10
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000107821
Entrez:   81621
UniGene:   Hs.632079
Chromosome Mapping:   10q24.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.350000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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