PrimePCR™ Probe Assay: FCN3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
Your Price:   Log In
Quantity:  
 

PrimePCR™ PreAmp for Probe Assay: FCN3, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: FCN3, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028896
Assay Design:   Intron-spanning
Chromosome Location:   1:27697384-27699712question
Amplicon Length:   136
Splice Variants Targeted:   ENST00000270879 ENST00000354982 ENST00000498393

Gene Information

Ficolins are a group of proteins which consist of a collagen-like domain and a fibrinogen-like domain. In human serum there are two types of ficolins both of which have lectin activity. The protein encoded by this gene is a thermolabile beta-2-macroglycoprotein found in all human serum and is a member of the ficolin/opsonin p35 lectin family. The protein which was initially identified based on its reactivity with sera from patients with systemic lupus erythematosus has been shown to have a calcium-independent lectin activity. The protein can activate the complement pathway in association with MASPs and sMAP thereby aiding in host defense through the activation of the lectin pathway. Alternative splicing occurs at this locus and two variants each encoding a distinct isoform have been identified. [provided by RefSeq Jul 2008]

Gene Symbol:   FCN3
Gene Name:   ficolin (collagen/fibrinogen domain containing) 3 (Hakata antigen)
Aliases:   FCNH, HAKA1, MGC22543
RefSeq:   NC_000001.10 NG_016279.1 NT_004610.19
Ensembl:   ENSG00000142748
Entrez:   8547
Chromosome Mapping:   1p36.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.000000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download
10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
10000088666 Instruction Manual, PrimePCR Assays, Panels, and Controls, Ver G Click to download
10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
3226 PIS_PrimePCR SARS-CoV-2 Single and Multiple Mutation Assays Click to download
10000147102 PrimePCR SARS-CoV-2 Multiple Mutation Assay Protocol Click to download