PrimePCR™ Probe Assay: SDHD, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028882
Assay Design:   Intron-spanning
Chromosome Location:   11:111957581-111958623question
Amplicon Length:   116
Splice Variants Targeted:   ENST00000375549 ENST00000528182 ENST00000528048 ENST00000528021

Gene Information

Complex II of the respiratory chain which is specifically involved in the oxidation of succinate carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The subunit D protein is one of two integral membrane proteins anchoring the complex to the matrix side of the membrane. Mutations in SDHD have been linked to hereditary paraganglioma. [provided by RefSeq Oct 2009]

Gene Symbol:   SDHD
Gene Name:   succinate dehydrogenase complex, subunit D, integral membrane protein
Aliases:   CBT1, PGL, PGL1, SDH4
RefSeq:   NC_000011.9 NG_012337.1 NT_033899.8
Ensembl:   ENSG00000204370
Entrez:   6392
UniGene:   Hs.356270
Chromosome Mapping:   11q23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 35.950000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download