PrimePCR™ Probe Assay: ACOX2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028853
Assay Design:   Intron-spanning
Chromosome Location:   3:58490864-58494649question
Amplicon Length:   150
Splice Variants Targeted:   ENST00000459701 ENST00000302819

Gene Information

The product of this gene belongs to the acyl-CoA oxidase family. It encodes the branched-chain acyl-CoA oxidase which is involved in the degradation of long branched fatty acids and bile acid intermediates in peroxisomes. Deficiency of this enzyme results in the accumulation of branched fatty acids and bile acid intermediates and may lead to Zellweger syndrome severe mental retardation and death in children. [provided by RefSeq Mar 2009]

Gene Symbol:   ACOX2
Gene Name:   acyl-CoA oxidase 2, branched chain
Aliases:   BCOX, BRCACOX, BRCOX, THCCox
RefSeq:   NC_000003.11 NT_022517.18
Ensembl:   ENSG00000168306
Entrez:   8309
UniGene:   Hs.444959
Chromosome Mapping:   3p14.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.996400
y-intercept 34.790000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download