PrimePCR™ Probe Assay: POU2F1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: POU2F1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: POU2F1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028850
Assay Design:   Intron-spanning
Chromosome Location:   1:167368529-167370731question
Amplicon Length:   88
Splice Variants Targeted:   ENST00000367866 ENST00000367865 ENST00000367862 ENST00000443275 ENST00000429375 ENST00000420254 ENST00000541643

Gene Information

The OCT1 transcription factor was among the first identified members of the POU transcription factor family (summarized by Sturm et al. 1993 [PubMed 8314572]). Members of this family contain the POU domain a 160-amino acid region necessary for DNA binding to the octameric sequence ATGCAAAT.[supplied by OMIM Jul 2010]

Gene Symbol:   POU2F1
Gene Name:   POU class 2 homeobox 1
Aliases:   FLJ42836, OCT1, OTF1
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000143190
Entrez:   5451
Chromosome Mapping:   1q24.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 36.260000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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