PrimePCR™ Probe Assay: HAS1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028764
Assay Design:   Intron-spanning
Chromosome Location:   19:52219597-52220277question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000540069 ENST00000222115

Gene Information

Hyaluronan or hyaluronic acid (HA) is a high molecular weight unbranched polysaccharide synthesized by a wide variety of organisms from bacteria to mammals and is a constituent of the extracellular matrix. It consists of alternating glucuronic acid and N-acetylglucosamine residues that are linked by beta-1-3 and beta-1-4 glycosidic bonds. HA is synthesized by membrane-bound synthase at the inner surface of the plasma membrane and the chains are extruded through pore-like structures into the extracellular space. It serves a variety of functions including space filling lubrication of joints and provision of a matrix through which cells can migrate. HA is actively produced during wound healing and tissue repair to provide a framework for ingrowth of blood vessels and fibroblasts. Changes in the serum concentration of HA are associated with inflammatory and degenerative arthropathies such as rheumatoid arthritis. In addition the interaction of HA with the leukocyte receptor CD44 is important in tissue-specific homing by leukocytes and overexpression of HA receptors has been correlated with tumor metastasis. HAS1 is a member of the newly identified vertebrate gene family encoding putative hyaluronan synthases and its amino acid sequence shows significant homology to the hasA gene product of Streptococcus pyogenes a glycosaminoglycan synthetase (DG42) from Xenopus laevis and a recently described murine hyaluronan synthase. [provided by RefSeq Jul 2008]

Gene Symbol:   HAS1
Gene Name:   hyaluronan synthase 1
Aliases:   HAS
RefSeq:   NC_000019.9 NT_011109.16
Ensembl:   ENSG00000105509
Entrez:   3036
UniGene:   Hs.57697
Chromosome Mapping:   19q13.4

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998500
y-intercept 33.820000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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