PrimePCR™ Probe Assay: IL27RA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: IL27RA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: IL27RA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028721
Assay Design:   Intron-spanning
Chromosome Location:   19:14153544-14157061question
Amplicon Length:   124
Splice Variants Targeted:   ENST00000263379

Gene Information

In mice CD4+ helper T-cells differentiate into type 1 (Th1) cells which are critical for cell-mediated immunity predominantly under the influence of IL12. Also IL4 influences their differentiation into type 2 (Th2) cells which are critical for most antibody responses. Mice deficient in these cytokines their receptors or associated transcription factors have impaired but are not absent of Th1 or Th2 immune responses. This gene encodes a protein which is similar to the mouse T-cell cytokine receptor Tccr at the amino acid level and is predicted to be a glycosylated transmembrane protein. [provided by RefSeq Jul 2008]

Gene Symbol:   IL27RA
Gene Name:   interleukin 27 receptor, alpha
Aliases:   CRL1, IL27R, TCCR, WSX1, zcytor1
RefSeq:   NC_000019.9 NT_011295.11
Ensembl:   ENSG00000104998
Entrez:   9466
Chromosome Mapping:   19p13.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999100
y-intercept 35.400000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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