PrimePCR™ Probe Assay: HTR3C, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028678
Assay Design:   Intron-spanning
Chromosome Location:   3:183772608-183774021question
Amplicon Length:   140
Splice Variants Targeted:   ENST00000318351

Gene Information

The product of this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit C of the type 3 receptor for 5-hydroxytryptamine (serotonin) a biogenic hormone that functions as a neurotransmitter a hormone and a mitogen. This receptor causes fast depolarizing responses in neurons after activation. Genes encoding subunits C D and E form a cluster on chromosome 3. [provided by RefSeq Jul 2008]

Gene Symbol:   HTR3C
Gene Name:   5-hydroxytryptamine (serotonin) receptor 3, family member C
Aliases:   Not Available
RefSeq:   NC_000003.11 NG_012749.1 NT_005612.16
Ensembl:   ENSG00000178084
Entrez:   170572
UniGene:   Hs.632579
Chromosome Mapping:   3q27.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.420000
Efficiency 97

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download