PrimePCR™ Probe Assay: STARD3NL, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $214.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028656
Assay Design:   Intron-spanning
Chromosome Location:   7:38247234-38254017question
Amplicon Length:   127
Splice Variants Targeted:   ENST00000009041 ENST00000434197 ENST00000396013 ENST00000440144 ENST00000453225 ENST00000429075 ENST00000544203

Gene Information

This gene encodes a late-endosomal protein that contains a conserved MENTAL (MLN64 N-terminal) domain. The encoded protein binds cholesterol molecules and may play a role in endosomal cholesterol transport through interactions with metastatic lymph node protein 64 (MLN64). [provided by RefSeq Sep 2011]

Gene Symbol:   STARD3NL
Gene Name:   STARD3 N-terminal like
Aliases:   MENTHO, MGC3251
RefSeq:   NC_000007.13 NT_007819.17
Ensembl:   ENSG00000010270
Entrez:   83930
UniGene:   Hs.309753
Chromosome Mapping:   7p14-p13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 34.920000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download