PrimePCR™ Probe Assay: MARK2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: MARK2, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MARK2, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028477
Assay Design:   Intron-spanning
Chromosome Location:   11:63662789-63666123question
Amplicon Length:   139
Splice Variants Targeted:   ENST00000508192 ENST00000361128 ENST00000350490 ENST00000502399 ENST00000543220 ENST00000540169 ENST00000509502 ENST00000513765 ENST00000408948 ENST00000425897 ENST00000402010 ENST00000315032 ENST00000377809 ENST00000413835 ENST00000508025 ENST00000535394 ENST00000377810

Gene Information

This gene encodes a member of the Par-1 family of serine/threonine protein kinases. The protein is an important regulator of cell polarity in epithelial and neuronal cells and also controls the stability of microtubules through phosphorylation and inactivation of several microtubule-associating proteins. The protein localizes to cell membranes. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Jul 2009]

Gene Symbol:   MARK2
Gene Name:   MAP/microtubule affinity-regulating kinase 2
Aliases:   EMK-1, EMK1, MGC99619, PAR-1, Par1b
RefSeq:   NC_000011.9 NT_167190.1
Ensembl:   ENSG00000072518
Entrez:   2011
Chromosome Mapping:   11q13

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 34.730000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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