PrimePCR™ Probe Assay: EPB41L1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: EPB41L1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: EPB41L1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028461
Assay Design:   Intron-spanning
Chromosome Location:   20:34763607-34765973question
Amplicon Length:   101
Splice Variants Targeted:   ENST00000202028 ENST00000406771 ENST00000430276 ENST00000452261 ENST00000373945 ENST00000338074 ENST00000373941 ENST00000373946 ENST00000397315 ENST00000441639 ENST00000397307 ENST00000344237

Gene Information

Erythrocyte membrane protein band 4.1 (EPB41) is a multifunctional protein that mediates interactions between the erythrocyte cytoskeleton and the overlying plasma membrane. The protein encoded by this gene is a neuronally-enriched protein that is structurally similar to EPB41. The encoded protein binds and stabilizes D2 and D3 dopamine receptors at the neuronal plasma membrane. Multiple transcript variants encoding different isoforms have been found for this gene but the full-length nature of only two of them has been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   EPB41L1
Gene Name:   erythrocyte membrane protein band 4.1-like 1
Aliases:   4.1N, DKFZp686H17242, KIAA0338, MGC11072
RefSeq:   NC_000020.10 NT_011362.10
Ensembl:   ENSG00000088367
Entrez:   2036
Chromosome Mapping:   20q11.2-q12

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998800
y-intercept 34.480000
Efficiency 103

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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