PrimePCR™ Probe Assay: ERI3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028423
Assay Design:   Intron-spanning
Chromosome Location:   1:44750507-44774041question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000372257 ENST00000372259 ENST00000456170 ENST00000452396 ENST00000433471 ENST00000372253 ENST00000537474

Gene Information

Description Not Available

Gene Symbol:   ERI3
Gene Name:   ERI1 exoribonuclease family member 3
Aliases:   FLJ22943, MGC2683, PINT1, PRNPIP
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000117419
Entrez:   79033
UniGene:   Hs.731413
Chromosome Mapping:   1p32

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997400
y-intercept 35.590000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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