PrimePCR™ Probe Assay: LTF, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028368
Assay Design:   Intron-spanning
Chromosome Location:   3:46495795-46496842question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000231751 ENST00000417439 ENST00000443496 ENST00000426532 ENST00000443743

Gene Information

This gene is a member of the transferrin family of genes and its protein product is found in the secondary granules of neutrophils. The protein is a major iron-binding protein in milk and body secretions with an antimicrobial activity making it an important component of the non-specific immune system. The protein demonstrates a broad spectrum of properties including regulation of iron homeostasis host defense against a broad range of microbial infections anti-inflammatory activity regulation of cellular growth and differentiation and protection against cancer development and metastasis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Nov 2010]

Gene Symbol:   LTF
Gene Name:   lactotransferrin
Aliases:   GIG12, HLF2, LF
RefSeq:   NC_000003.11 NG_023257.1 NT_022517.18
Ensembl:   ENSG00000012223
Entrez:   4057
UniGene:   Hs.529517
Chromosome Mapping:   3p21.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 35.370000
Efficiency 94

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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