PrimePCR™ Probe Assay: KAZN, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: KAZN, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: KAZN, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028359
Assay Design:   Intron-spanning
Chromosome Location:   1:15386769-15392138question
Amplicon Length:   63
Splice Variants Targeted:   ENST00000376030 ENST00000503743 ENST00000361144 ENST00000400797 ENST00000422387 ENST00000400798

Gene Information

This gene encodes a protein that plays a role in desmosome assembly cell adhesion cytoskeletal organization and epidermal differentiation. This protein co-localizes with desmoplakin and the cytolinker protein periplakin. In general this protein localizes to the nucleus desmosomes cell membrane and cortical actin-based structures. Some isoforms of this protein also associate with microtubules. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional splice variants have been described but their biological validity has not been verified. [provided by RefSeq Aug 2011]

Gene Symbol:   KAZN
Gene Name:   kazrin, periplakin interacting protein
Aliases:   DKFZp686D08115, FLJ10199, FLJ32593, FLJ43398, FLJ43806, KAZ, KIAA1026, MGC126686, MGC142181
RefSeq:   NC_000001.10 NT_004610.19
Ensembl:   ENSG00000189337
Entrez:   23254
Chromosome Mapping:   1p36.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 35.160000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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