PrimePCR™ Probe Assay: CDK5RAP2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028323
Assay Design:   Intron-spanning
Chromosome Location:   9:123177393-123182183question
Amplicon Length:   133
Splice Variants Targeted:   ENST00000349780 ENST00000360190 ENST00000416449 ENST00000425647 ENST00000360822 ENST00000359309 ENST00000345313

Gene Information

Neuronal CDC2-like kinase which is involved in the regulation of neuronal differentiation is composed of a catalytic subunit CDK5 and an activating subunit p25NCK5A. The protein encoded by this gene binds to p25NCK5A and therefore may be involved in neuronal differentiation. The encoded protein may also be a substrate of neuronal CDC2-like kinase. Multiple transcript variants exist for this gene but the full-length nature of only two has been determined. [provided by RefSeq Jul 2008]

Gene Symbol:   CDK5RAP2
Gene Name:   CDK5 regulatory subunit associated protein 2
Aliases:   C48, Cep215, DKFZp686B1070, DKFZp686D1070, KIAA1633, MCPH3
RefSeq:   NC_000009.11 NT_008470.19 NG_008999.1
Ensembl:   ENSG00000136861
Entrez:   55755
Chromosome Mapping:   9q33.2

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.360000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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