PrimePCR™ Probe Assay: MED21, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028297
Assay Design:   Intron-spanning
Chromosome Location:   12:27179353-27180337question
Amplicon Length:   143
Splice Variants Targeted:   ENST00000546323 ENST00000282892

Gene Information

The Mediator is a multiprotein coactivator that is required by DNA-binding transcription factors for transcriptional activation of polymerase II (see MIM 180660)-transcribed genes. MED21 is a Mediator subunit predicted to reside in the middle module and appears to be a core subunit found in nearly all Mediator preparations (summary by Sato et al. 2004 [PubMed 15175163]).[supplied by OMIM Nov 2010]

Gene Symbol:   MED21
Gene Name:   mediator complex subunit 21
Aliases:   SRB7, SURB7
RefSeq:   NC_000012.11 NT_009714.17
Ensembl:   ENSG00000152944
Entrez:   9412
UniGene:   Hs.286145
Chromosome Mapping:   12p11.23

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.600000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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