PrimePCR™ Probe Assay: DIP2C, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028268
Assay Design:   Intron-spanning
Chromosome Location:   10:412316-415488question
Amplicon Length:   61
Splice Variants Targeted:   ENST00000280886 ENST00000421992 ENST00000540204 ENST00000381496

Gene Information

This gene encodes a member of the disco-interacting protein homolog 2 family. The protein shares strong similarity with a Drosophila protein which interacts with the transcription factor disco and is expressed in the nervous system. [provided by RefSeq Oct 2008]

Gene Symbol:   DIP2C
Gene Name:   DIP2 disco-interacting protein 2 homolog C (Drosophila)
Aliases:   FLJ34444, FLJ44075, KIAA0934
RefSeq:   NC_000010.10 NT_008705.16
Ensembl:   ENSG00000151240
Entrez:   22982
UniGene:   Hs.432397
Chromosome Mapping:   10p15.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 34.290000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download