PrimePCR™ Probe Assay: KLHL20, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028252
Assay Design:   Intron-spanning
Chromosome Location:   1:173726242-173735375question
Amplicon Length:   118
Splice Variants Targeted:   ENST00000209884 ENST00000546011

Gene Information

The protein encoded by this gene is a member of the kelch family of proteins which is characterized by a 44-56 amino acid repeat motif. The kelch motif appears in many different polypeptide contexts and contains multiple potential protein-protein contact sites. Members of this family are present both throughout the cell and extracellularly with diverse activities. [provided by RefSeq Jul 2008]

Gene Symbol:   KLHL20
Gene Name:   kelch-like 20 (Drosophila)
Aliases:   KHLHX, KLEIP, KLHLX, RP3-383J4.3
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000076321
Entrez:   27252
UniGene:   Hs.495035
Chromosome Mapping:   1q25.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.210000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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