PrimePCR™ Probe Assay: ZDHHC16, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028154
Assay Design:   Intron-spanning
Chromosome Location:   10:99215751-99216598question
Amplicon Length:   86
Splice Variants Targeted:   ENST00000370854 ENST00000370846 ENST00000352634 ENST00000353979 ENST00000370842 ENST00000345745 ENST00000420089 ENST00000417044 ENST00000393760

Gene Information

Description Not Available

Gene Symbol:   ZDHHC16
Gene Name:   zinc finger, DHHC-type containing 16
Aliases:   APH2, MGC2993
RefSeq:   NC_000010.10 NT_030059.13
Ensembl:   ENSG00000171307
Entrez:   84287
UniGene:   Hs.76662
Chromosome Mapping:   10q24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.470000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download