PrimePCR™ Probe Assay: KHSRP, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0028079
Assay Design:   Intron-spanning
Chromosome Location:   19:6416998-6417790question
Amplicon Length:   112
Splice Variants Targeted:   ENST00000201886 ENST00000398148

Gene Information

The KHSRP gene encodes a multifunctional RNA-binding protein implicated in a variety of cellular processes including transcription alternative pre-mRNA splicing and mRNA localization (Min et al. 1997 [PubMed 9136930]; Gherzi et al. 2004 [PubMed 15175153]).[supplied by OMIM Apr 2010]

Gene Symbol:   KHSRP
Gene Name:   KH-type splicing regulatory protein
Aliases:   FBP2, FUBP2, KSRP, MGC99676
RefSeq:   NC_000019.9 NT_011255.14
Ensembl:   ENSG00000088247
Entrez:   8570
UniGene:   Hs.727344
Chromosome Mapping:   19p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 32.900000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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