PrimePCR™ Probe Assay: CACNG1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027989
Assay Design:   Intron-spanning
Chromosome Location:   17:65051320-65052252question
Amplicon Length:   99
Splice Variants Targeted:   ENST00000226021

Gene Information

Voltage-dependent calcium channels are composed of five subunits. The protein encoded by this gene represents one of these subunits gamma and is one of two known gamma subunit proteins. This particular gamma subunit is part of skeletal muscle 14-dihydropyridine-sensitive calcium channels and is an integral membrane protein that plays a role in excitation-contraction coupling. This gene is part of a functionally diverse eight-member protein subfamily of the PMP-22/EMP/MP20 family and is located in a cluster with two family members that function as transmembrane AMPA receptor regulatory proteins (TARPs). [provided by RefSeq Dec 2010]

Gene Symbol:   CACNG1
Gene Name:   calcium channel, voltage-dependent, gamma subunit 1
Aliases:   CACNLG
RefSeq:   NC_000017.10 NT_010783.15
Ensembl:   ENSG00000108878
Entrez:   786
UniGene:   Hs.147989
Chromosome Mapping:   17q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999500
y-intercept 33.050000
Efficiency 101

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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