This is the amplicon context sequence in accordance with the minimum information for the publication of real-time quantitative PCR experiements (MIQE) guidelines. For more details, please refer to the following publication, "Primer Sequence Disclosure: A Clarification of the MIQE Guidelines."
Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.
PrimePCR™ PreAmp for Probe Assay: MAPK3, Human
PrimePCR™ Template for Probe Assay: MAPK3, Human
The protein encoded by this gene is a member of the MAP kinase family. MAP kinases also known as extracellular signal-regulated kinases (ERKs) act in a signaling cascade that regulates various cellular processes such as proliferation differentiation and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq Jul 2008]
Products used to generate validation data:
Amplification of cDNA generated from universal RNA.
Melt curve analysis of above amplification.
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.