PrimePCR™ Probe Assay: MECOM, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: MECOM, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: MECOM, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027711
Assay Design:   Intron-spanning
Chromosome Location:   3:168845810-168849279question
Amplicon Length:   72
Splice Variants Targeted:   ENST00000464456 ENST00000472280 ENST00000494292 ENST00000468789 ENST00000460814 ENST00000433243 ENST00000484519 ENST00000460890 ENST00000487503 ENST00000494597 ENST00000475754 ENST00000481315 ENST00000264674 ENST00000392736

Gene Information

The protein encoded by this gene is a transcriptional regulator and oncoprotein that may be involved in hematopoiesis apoptosis development and cell differentiation and proliferation. The encoded protein can interact with CTBP1 SMAD3 CREBBP KAT2B MAPK8 and MAPK9. This gene can undergo translocation with the AML1 gene resulting in overexpression of this gene and the onset of leukemia. Several transcript variants encoding a few different isoforms have been found for this gene. [provided by RefSeq Mar 2011]

Gene Symbol:   MECOM
Gene Name:   MDS1 and EVI1 complex locus
Aliases:   AML1-EVI-1, EVI1, MDS1, MDS1-EVI1, MGC163392, MGC97004, PRDM3
RefSeq:   NC_000003.11 NT_005612.16
Ensembl:   ENSG00000085276
Entrez:   2122
Chromosome Mapping:   3q26

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.020000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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