PrimePCR™ Probe Assay: LAMA1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027686
Assay Design:   Intron-spanning
Chromosome Location:   18:6958540-6959380question
Amplicon Length:   133
Splice Variants Targeted:   ENST00000389658 ENST00000344342

Gene Information

Description Not Available

Gene Symbol:   LAMA1
Gene Name:   laminin, alpha 1
Aliases:   LAMA, S-LAM-alpha
RefSeq:   NC_000018.9 NT_010859.14
Ensembl:   ENSG00000101680
Entrez:   284217
UniGene:   Hs.270364
Chromosome Mapping:   18p11.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999700
y-intercept 35.320000
Efficiency 96

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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