PrimePCR™ Probe Assay: SYNCRIP, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027533
Assay Design:   Intron-spanning
Chromosome Location:   6:86333764-86346779question
Amplicon Length:   132
Splice Variants Targeted:   ENST00000355238 ENST00000369622

Gene Information

This gene encodes a member of the cellular heterogeneous nuclear ribonucleoprotein (hnRNP) family. hnRNPs are RNA binding proteins that complex with heterogeneous nuclear RNA (hnRNA) and regulate alternative splicing polyadenylation and other aspects of mRNA metabolism and transport. The encoded protein plays a role in multiple aspects of mRNA maturation and is associated with several multiprotein complexes including the apoB RNA editing-complex and survival of motor neurons (SMN) complex. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene and a pseudogene of this gene is located on the short arm of chromosome 20. [provided by RefSeq Dec 2011]

Gene Symbol:   SYNCRIP
Gene Name:   synaptotagmin binding, cytoplasmic RNA interacting protein
Aliases:   FLJ31626, GRY-RBP, GRYRBP, HNRPQ1, NSAP1, PP68, dJ3J17.2, hnRNP-Q
RefSeq:   NC_000006.11 NT_007299.13
Ensembl:   ENSG00000135316
Entrez:   10492
UniGene:   Hs.571177
Chromosome Mapping:   6q14-q15

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999900
y-intercept 35.520000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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