PrimePCR™ Probe Assay: ADPGK, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027390
Assay Design:   Intron-spanning
Chromosome Location:   15:73048718-73052832question
Amplicon Length:   126
Splice Variants Targeted:   ENST00000311669 ENST00000443764 ENST00000331065

Gene Information

ADPGK (EC 2.7.1.147) catalyzes the ADP-dependent phosphorylation of glucose to glucose-6-phosphate and may play a role in glycolysis possibly during ischemic conditions (Ronimus and Morgan 2004 [PubMed 14975750]).[supplied by OMIM Mar 2008]

Gene Symbol:   ADPGK
Gene Name:   ADP-dependent glucokinase
Aliases:   2610017G09Rik, ADP-GK, DKFZp434B195
RefSeq:   NC_000015.9 NT_010194.17
Ensembl:   ENSG00000159322
Entrez:   83440
UniGene:   Hs.730668
Chromosome Mapping:   15q24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 35.290000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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