PrimePCR™ Probe Assay: ZNF330, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027295
Assay Design:   Intron-spanning
Chromosome Location:   4:142147923-142150806question
Amplicon Length:   130
Splice Variants Targeted:   ENST00000262990 ENST00000512809 ENST00000503649 ENST00000512738 ENST00000421169

Gene Information

Description Not Available

Gene Symbol:   ZNF330
Gene Name:   zinc finger protein 330
Aliases:   HSA6591, NOA36
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000109445
Entrez:   27309
UniGene:   Hs.120766
Chromosome Mapping:   4q31.21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998200
y-intercept 36.510000
Efficiency 91

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download