PrimePCR™ Probe Assay: SCN9A, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: SCN9A, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: SCN9A, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027256
Assay Design:   Intron-spanning
Chromosome Location:   2:167108279-167128956question
Amplicon Length:   135
Splice Variants Targeted:   ENST00000409672 ENST00000409435 ENST00000375387 ENST00000303354

Gene Information

This gene encodes a voltage-gated sodium channel which plays a significant role in nociception signaling. Mutations in this gene have been associated with primary erythermalgia channelopathy-associated insensitivity to pain and paroxysmal extreme pain disorder. [provided by RefSeq Aug 2009]

Gene Symbol:   SCN9A
Gene Name:   sodium channel, voltage-gated, type IX, alpha subunit
Aliases:   ETHA, FEB3B, NE-NA, NENA, Nav1.7, PN1
RefSeq:   NC_000002.11 NG_012798.1 NT_005403.17
Ensembl:   ENSG00000169432
Entrez:   6335
Chromosome Mapping:   2q24

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 35.480000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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