PrimePCR™ Probe Assay: SCARB1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027176
Assay Design:   Intron-spanning
Chromosome Location:   12:125292446-125296432question
Amplicon Length:   130
Splice Variants Targeted:   ENST00000339570 ENST00000415380 ENST00000261693 ENST00000546215 ENST00000544327 ENST00000376788 ENST00000540495 ENST00000541205

Gene Information

The protein encoded by this gene is a plasma membrane receptor for high density lipoprotein cholesterol (HDL). The encoded protein mediates cholesterol transfer to and from HDL. In addition this protein is a receptor for hepatitis C virus glycoprotein E2. Two transcript variants encoding different isoforms have been found for this gene.[provided by RefSeq Mar 2011]

Gene Symbol:   SCARB1
Gene Name:   scavenger receptor class B, member 1
Aliases:   CD36L1, CLA-1, CLA1, HDLQTL6, MGC138242, SR-BI, SRB1
RefSeq:   NC_000012.11 NT_009755.19
Ensembl:   ENSG00000073060
Entrez:   949
UniGene:   Hs.731377
Chromosome Mapping:   12q24.31

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 34.740000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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