PrimePCR™ Probe Assay: BPNT1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: BPNT1, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: BPNT1, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027146
Assay Design:   Intron-spanning
Chromosome Location:   1:220247340-220253121question
Amplicon Length:   97
Splice Variants Targeted:   ENST00000354807 ENST00000463953 ENST00000498791 ENST00000498237 ENST00000302686 ENST00000322067 ENST00000414869 ENST00000469520

Gene Information

BPNT1 also called bisphosphate 3-prime-nucleotidase or BPntase is a member of a magnesium-dependent phosphomonoesterase family. Lithium a major drug used to treat manic depression acts as an uncompetitive inhibitor of BPntase. The predicted human protein is 92% identical to mouse BPntase. BPntase's physiologic role in nucleotide metabolism may be regulated by inositol signaling pathways. The inhibition of human BPntase may account for lithium-induced nephrotoxicity. [provided by RefSeq Jul 2008]

Gene Symbol:   BPNT1
Gene Name:   3'(2'), 5'-bisphosphate nucleotidase 1
Aliases:   PIP
RefSeq:   NC_000001.10 NT_167186.1
Ensembl:   ENSG00000162813
Entrez:   10380
Chromosome Mapping:   1q41

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999300
y-intercept 34.260000
Efficiency 104

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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