PrimePCR™ Probe Assay: HERC6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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PrimePCR™ PreAmp for Probe Assay: HERC6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: HERC6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027142
Assay Design:   Intron-spanning
Chromosome Location:   4:89314640-89317228question
Amplicon Length:   127
Splice Variants Targeted:   ENST00000380265 ENST00000264346 ENST00000438983 ENST00000511939 ENST00000273960

Gene Information

HERC6 belongs to the HERC family of ubiquitin ligases all of which contain a HECT domain and at least 1 RCC1 (MIM 179710)-like domain (RLD). The 350-amino acid HECT domain is predicted to catalyze the formation of a thioester with ubiquitin before transferring it to a substrate and the RLD is predicted to act as a guanine nucleotide exchange factor for small G proteins (Hochrainer et al. 2005 [PubMed 15676274]).[supplied by OMIM Mar 2008]

Gene Symbol:   HERC6
Gene Name:   hect domain and RLD 6
Aliases:   FLJ20637
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000138642
Entrez:   55008
Chromosome Mapping:   4q22.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999200
y-intercept 35.000000
Efficiency 98

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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