PrimePCR™ Probe Assay: MCOLN2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027093
Assay Design:   Intron-spanning
Chromosome Location:   1:85405250-85406597question
Amplicon Length:   142
Splice Variants Targeted:   ENST00000370608 ENST00000284027

Gene Information

Mucolipins constitute a family of cation channel proteins with homology to the transient receptor potential superfamily. In mammals the mucolipin family includes 3 members MCOLN1 (MIM 605248) MCOLN2 and MCOLN3 (MIM 607400) that exhibit a common 6-membrane-spanning topology. Homologs of mammalian mucolipins exist in Drosophila and C. elegans. Mutations in the human MCOLN1 gene cause mucolipodosis IV (MIM 262650) (Karacsonyi et al. 2007 [PubMed 17662026]).[supplied by OMIM Sep 2009]

Gene Symbol:   MCOLN2
Gene Name:   mucolipin 2
Aliases:   FLJ36691, TRP-ML2, TRPML2
RefSeq:   NC_000001.10 NT_032977.9
Ensembl:   ENSG00000153898
Entrez:   255231
UniGene:   Hs.591446
Chromosome Mapping:   1p22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998900
y-intercept 34.680000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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