PrimePCR™ Probe Assay: MDGA2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0027066
Assay Design:   Intron-spanning
Chromosome Location:   14:47530641-47566089question
Amplicon Length:   144
Splice Variants Targeted:   ENST00000399232 ENST00000357362 ENST00000554762 ENST00000439988 ENST00000426342

Gene Information

Description Not Available

Gene Symbol:   MDGA2
Gene Name:   MAM domain containing glycosylphosphatidylinositol anchor 2
Aliases:   MAMDC1, c14_5286
RefSeq:   NC_000014.8 NT_026437.12
Ensembl:   ENSG00000139915
Entrez:   161357
UniGene:   Hs.436380
Chromosome Mapping:   14q21.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999400
y-intercept 35.160000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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