PrimePCR™ Probe Assay: OAS1, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $255.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026992
Assay Design:   Intron-spanning
Chromosome Location:   12:113344987-113346427question
Amplicon Length:   95
Splice Variants Targeted:   ENST00000202917 ENST00000445409 ENST00000452357 ENST00000549820 ENST00000551241 ENST00000553185 ENST00000550689 ENST00000377508

Gene Information

This gene encodes a member of the 2-5A synthetase family essential proteins involved in the innate immune response to viral infection. The encoded protein is induced by interferons and uses adenosine triphosphate in 2'-specific nucleotidyl transfer reactions to synthesize 2'5'-oligoadenylates (2-5As). These molecules activate latent RNase L which results in viral RNA degradation and the inhibition of viral replication. The three known members of this gene family are located in a cluster on chromosome 12. Mutations in this gene have been associated with host susceptibility to viral infection. Alternatively spliced transcript variants encoding different isoforms have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   OAS1
Gene Name:   2',5'-oligoadenylate synthetase 1, 40/46kDa
Aliases:   IFI-4, OIAS, OIASI
RefSeq:   NC_000012.11 NT_009775.17 NG_011530.1
Ensembl:   ENSG00000089127
Entrez:   4938
Chromosome Mapping:   12q24.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 34.800000
Efficiency 105

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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