PrimePCR™ Probe Assay: FRS3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026900
Assay Design:   Intron-spanning
Chromosome Location:   6:41740596-41743232question
Amplicon Length:   148
Splice Variants Targeted:   ENST00000373018 ENST00000426290 ENST00000259748

Gene Information

The protein encoded by this gene is a substrate for the fibroblast growth factor receptor. It is found in peripheral plasma membrane and functions in linking FGF receptor stimulation to activators of Ras. [provided by RefSeq Jul 2008]

Gene Symbol:   FRS3
Gene Name:   fibroblast growth factor receptor substrate 3
Aliases:   FRS2B, FRS2beta, MGC17167, SNT-2, SNT2
RefSeq:   NC_000006.11 NT_007592.15
Ensembl:   ENSG00000137218
Entrez:   10817
UniGene:   Hs.194208
Chromosome Mapping:   6p21.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 34.860000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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