PrimePCR™ Probe Assay: BMP3, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026894
Assay Design:   Intron-spanning
Chromosome Location:   4:81967702-81974568question
Amplicon Length:   141
Splice Variants Targeted:   ENST00000282701

Gene Information

BMP3 belongs to the transforming growth factor-beta (TGFB) superfamily. Bone morphogenic protein also known as osteogenin induces bone formation. [provided by RefSeq Jul 2008]

Gene Symbol:   BMP3
Gene Name:   bone morphogenetic protein 3
Aliases:   BMP-3A
RefSeq:   NC_000004.11 NT_016354.19
Ensembl:   ENSG00000152785
Entrez:   651
UniGene:   Hs.387411
Chromosome Mapping:   4q21

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 34.580000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download