PrimePCR™ Probe Assay: IL28RA, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: IL28RA, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: IL28RA, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026864
Assay Design:   Intron-spanning
Chromosome Location:   1:24488096-24495960question
Amplicon Length:   103
Splice Variants Targeted:   ENST00000327535 ENST00000327575 ENST00000374421 ENST00000374419 ENST00000374418

Gene Information

The protein encoded by this gene belongs to the class II cytokine receptor family. This protein forms a receptor complex with interleukine 10 receptor beta (IL10RB). The receptor complex has been shown to interact with three closely related cytokines including interleukin 28A (IL28A) interleukin 28B (IL28B) and interleukin 29 (IL29). The expression of all three cytokines can be induced by viral infection. The cells overexpressing this protein have been found to have enhanced responses to IL28A and IL29 but decreased response to IL28B. Three alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq Jul 2008]

Gene Symbol:   IL28RA
Gene Name:   interleukin 28 receptor, alpha (interferon, lambda receptor)
Aliases:   CRF2/12, IFNLR, IFNLR1, IL-28R1, LICR2
RefSeq:   NC_000001.10 NT_004610.19
Ensembl:   ENSG00000185436
Entrez:   163702
Chromosome Mapping:   1p36.11

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999600
y-intercept 35.680000
Efficiency 99

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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10039761 PrimePCR™ Assays Quick Guide, Ver B Click to download
10042030 PrimePCR™ PreAmp Assay Quick Guide, Rev A Click to download
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10000143205 SARS-CoV-2 Variant Detection RT-PCR Assay Protocol Click to download
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