PrimePCR™ Probe Assay: DSTYK, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
Your Price:   Log In
 

Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026799
Assay Design:   Intron-spanning
Chromosome Location:   1:205138927-205156637question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000367161 ENST00000367162 ENST00000367160

Gene Information

This gene encodes a dual serine/threonine and tyrosine protein kinase which is expressed in multiple tissues. It is thought to function as a regulator of cell death. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq Dec 2008]

Gene Symbol:   DSTYK
Gene Name:   dual serine/threonine and tyrosine protein kinase
Aliases:   DustyPK, KIAA0472, RIP5, RIPK5
RefSeq:   NC_000001.10 NT_004487.19
Ensembl:   ENSG00000133059
Entrez:   25778
UniGene:   Hs.6874
Chromosome Mapping:   1q32.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998100
y-intercept 36.360000
Efficiency 95

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Number Description Download