PrimePCR™ Probe Assay: HCN2, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026797
Assay Design:   Intron-spanning
Chromosome Location:   19:603928-605134question
Amplicon Length:   84
Splice Variants Targeted:   ENST00000251287

Gene Information

Hyperpolarization-activated cation channels of the HCN gene family such as HCN2 contribute to spontaneous rhythmic activity in both heart and brain.[supplied by OMIM Jul 2010]

Gene Symbol:   HCN2
Gene Name:   hyperpolarization activated cyclic nucleotide-gated potassium channel 2
Aliases:   BCNG-2, BCNG2, HAC-1
RefSeq:   NC_000019.9 NG_023049.1 NT_011255.14
Ensembl:   ENSG00000099822
Entrez:   610
UniGene:   Hs.124161
Chromosome Mapping:   19p13.3

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997700
y-intercept 36.140000
Efficiency 100

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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