PrimePCR™ Probe Assay: EGFL6, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

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PrimePCR™ PreAmp for Probe Assay: EGFL6, Human
Reaction:     400 reactions
Gene-specific PCR primers for the unbiased preamplification of small quantities of cDNA for subsequent use in downstream gene expression analysis.

PrimePCR™ Template for Probe Assay: EGFL6, Human
Reaction:     200 x 20 µl reactions
Gene-specific synthetic DNA template designed to give a positive real-time PCR result when used with the corresponding probe assay.


Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026613
Assay Design:   Intron-spanning
Chromosome Location:   X:13626545-13635949question
Amplicon Length:   92
Splice Variants Targeted:   ENST00000361306 ENST00000380602

Gene Information

This gene encodes a member of the epidermal growth factor (EGF) repeat superfamily. Members of this superfamily are characterized by the presence of EGF-like repeats and are often involved in the regulation of cell cycle proliferation and developmental processes. The gene product contains a signal peptide suggesting that it is secreted; an EGF repeat region consisting of 4 complete EGF-like repeats and 1 partial EGF-like repeat 3 of which have a calcium-binding consensus sequence; an arg-gly-asp integrin association motif; and a MAM domain which is believed to have an adhesive function. This gene is expressed early during development and its expression has been detected in lung and meningioma tumors. [provided by RefSeq Jul 2008]

Gene Symbol:   EGFL6
Gene Name:   EGF-like-domain, multiple 6
Aliases:   DKFZp564P2063, MAEG, W80
RefSeq:   NC_000023.10 NT_167197.1 NG_015963.1
Ensembl:   ENSG00000198759
Entrez:   25975
Chromosome Mapping:   Xp22

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)

Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.997800
y-intercept 34.280000
Efficiency 107

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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