PrimePCR™ Probe Assay: IFT88, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026346
Assay Design:   Intron-spanning
Chromosome Location:   13:21189968-21199963question
Amplicon Length:   96
Splice Variants Targeted:   ENST00000389374 ENST00000351808 ENST00000319980 ENST00000382778 ENST00000537103

Gene Information

This gene encodes a member of the tetratrico peptide repeat (TPR) family. Mutations of a similar gene in mouse can cause polycystic kidney disease. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq Jul 2008]

Gene Symbol:   IFT88
Gene Name:   intraflagellar transport 88 homolog (Chlamydomonas)
Aliases:   D13S1056E, DAF19, MGC26259, TG737, TTC10, hTg737
RefSeq:   NC_000013.10 NT_024524.14
Ensembl:   ENSG00000032742
Entrez:   8100
UniGene:   Hs.187376
Chromosome Mapping:   13q12.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.998600
y-intercept 34.710000
Efficiency 102

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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