PrimePCR™ Probe Assay: GAS7, Human

RT

Real-time PCR probe assay designed for gene expression analysis. Probe assays consist of unlabeled PCR primers and a dual labeled fluorescent probe.

List Price:    $202.00
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Assay Information

Technology:   qPCR
Assay Type:   Probe
Application:   Gene Expression
Unique Assay ID:   qHsaCIP0026316
Assay Design:   Intron-spanning
Chromosome Location:   17:9837487-9846450question
Amplicon Length:   132
Splice Variants Targeted:   ENST00000323816 ENST00000396115 ENST00000537970 ENST00000437099 ENST00000432992 ENST00000541114 ENST00000542249

Gene Information

Growth arrest-specific 7 is expressed primarily in terminally differentiated brain cells and predominantly in mature cerebellar Purkinje neurons. GAS7 plays a putative role in neuronal development. Several transcript variants encoding proteins which vary in the N-terminus have been described. [provided by RefSeq Jul 2008]

Gene Symbol:   GAS7
Gene Name:   growth arrest-specific 7
Aliases:   KIAA0394, MGC1348, MLL/GAS7
RefSeq:   NC_000017.10 NT_010718.16
Ensembl:   ENSG00000007237
Entrez:   8522
UniGene:   Hs.462214
Chromosome Mapping:   17p13.1

The below validation information is for the Primer Pair only   Download Validation Data (.pdf)


Products used to generate validation data:

Real-Time PCR Instrument CFX384 Real-Time PCR Detection System
Reverse Transcription Reagent iScript™ Advanced cDNA Synthesis Kit for RT-qPCR
Real-Time PCR Supermix SsoAdvanced™ SYBR® Green Supermix
Experimental Sample qPCR Human Reference Total RNA

Summary Data:

R2 0.999000
y-intercept 36.250000
Efficiency 93

Amplification Plot
Amplification of cDNA generated from universal RNA.

Amplification of cDNA generated from universal RNA.

Melt Peak
Melt curve analysis of above amplification.

Melt curve analysis of above amplification.

Standard Curve
Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

Standard curve generated using 20 million copies of template diluted 10 fold to 20 copies.

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